Direct Evidence on the Contribution of a Missense Mutation in GDF9 to Variation in Ovulation Rate of Finnsheep

peer-reviewedThe Finnish Landrace (Finnsheep) is a well known high-prolificacy sheep breed and has been used in many countries as a source of genetic material to increase fecundity of local breeds. Analyses to date have indicated that mutations with a large effect on ovulation rate are not responsible for the exceptional prolificacy of Finnsheep. The objectives of this study were to ascertain if: 1) any of 12 known mutations with large effects on ovulation rate in sheep, or 2) any other DNA sequence variants within the candidate genes GDF9 and BMP15 are implicated in the high prolificacy of the Finnish Landrace breed; using material from lines developed by divergent selection on ovulation rate. Genotyping results showed that none of 12 known mutations (FecBB, FecXB, FecXG, FecXGR, FecXH, FecXI, FecXL, FecXO, FecXR, FecGE, FecGH, or FecGT) were present in a sample of 108 Finnsheep and, thus, do not contribute to the exceptional prolificacy of the breed. However, DNA sequence analysis of GDF9 identified a previously known mutation, V371M, whose frequency differed significantly (P<0.001) between High and Low ovulation rate lines. While analysis of ovulation rate data for Finnsheep failed to establish a significant association between this trait and V371M, analysis of data on Belclare sheep revealed a significant association between V371M and ovulation rate (P<0.01). Ewes that were heterozygous for V371M exhibited increased ovulation rate (+0.17, s.e. 0.080; P<0.05) compared to wild type and the effect was non-additive (ovulation rate of heterozygotes was significantly lower (P<0.01) than the mean of the homozygotes). This finding brings to 13 the number of mutations that have large effects on ovulation rate in sheep and to 5, including FecBB, FecGE, FecXO and FecXGR, the number of mutations within the TGFβ superfamily with a positive effect on prolificacy in the homozygous state

On the Possibility of Authentic Christian Spirituality In The Post-Critical Age.

This project consists of three of my books (plus 2 chapters in another) and a summary statement on the topic entitled above. The books are: Being Saved (London, SCM 1985); Death Be Not Proud (London, Collins Fount 1989); Reason To Believe (London, Sinclair-Stevenson 1995); and the chapters appear in Anderson and Mullen ed; Faking It: The Sentimentalising of Society (London, The St Edmundsbury Press 1998). In Being Saved I made an extended comparison between the doctrines of traditional Christianity and the psychological theory C.G. Jung showing how these systems can cross-reference and cross-fertilise each other; and concluding that authentic spirituality can be enriched by such a comparison, but explicitly not concluding that Christian doctrine can be reduced to Jungian terms. In Death Be Not Proud I attempted a phenomenological study of the idea and experience of death and considered how this may be approached from the point of our awareness of the certainty of our own death and from the point of the bereaved. The book includes a sympathetic reflection on suicide and an argument for the trith of the doctrine of the resurrection to eternal life. Reason To Believe is a book of apologetics for the principal doctrines of Christianity as found in the Apostles' Creed, an argument for traditional texts in religious education and worship and a defence of the institutional church. In Faking It evidenced the widespread sentimentality in much contemporary worship and religious teaching and I identified this as an example of sentimentality which, religiously applied, I identified as inauthentic spirituality. The works show a continuity and development of though supported by a considerable project of reading and reflection which can be traced in the notes and bibliography

The Collaborative Development of New CFD Methods Adapted for Tilt Rotor Aircraft in the HiPerTilt Project

No abstract available

A note on the use of FTA™ technology for storage of blood samples for DNA analysis and removal of PCR inhibitors

peer-reviewedFTA™ technology is widely used across many molecular disciplines for sample capture, storage and analysis. The use of this technology for the long-term storage of blood samples for DNA analysis was examined as well as its potential to remove inhibitors from DNA samples previously extracted from blood with PCR inhibitors remaining. It was found that blood spots stored on FTA™ cards for 8 years at room temperature gave successful PCR products and that FTA™ cards are a useful tool for removing substances in samples which interfere with or inhibit, the PCR reaction

Multicomputer communication system

A local area network is provided for a plurality of autonomous computers which operate at different rates and under different protocols coupled by network bus adapters to a global bus. A host computer (HC) divides a message file to be transmitted into blocks, each with a header that includes a data type identifier and a trailer. The associated network bus adapter (NBA) then divides the data into packets, each with a header to which a transport header and trailer is added with frame type code which specifies one of three modes of addressing in the transmission of data, namely a physical address mode for computer to computer transmission using two bytes for source and destination addresses, a logical address mode and a data type mode. In the logical address mode, one of the two addressing bytes contains a logical channel number (LCN) established between the transmitting and one or more receiving computers. In the data type mode, one of the addressing bytes contains a code identifying the type of data

Investigation of Prolific Sheep from UK and Ireland for Evidence on Origin of the Mutations in BMP15 (FecXG, FecXB) and GDF9 (FecGH) in Belclare and Cambridge Sheep

peer-reviewedThis paper concerns the likely origin of three mutations with large effects on ovulation rate identified in the Belclare and Cambridge sheep breeds; two in the BMP15 gene (FecXG and FecXB) and the third (FecGH) in GDF9. All three mutations segregate in Belclare sheep while one, FecXB, has not been found in the Cambridge. Both Belclare and Cambridge breeds are relatively recently developed composites that have common ancestry through the use of genetic material from the Finnish Landrace and Lleyn breeds. The development of both composites also involved major contributions from exceptionally prolific ewes screened from flocks in Ireland (Belclare) and Britain (Cambridge) during the 1960s. The objective of the current study was to establish the likely origin of the mutations (FecXG, FecXB and FecGH) through analysis of DNA from Finnish Landrace and Lleyn sheep, and Galway and Texel breeds which contributed to the development of the Belclare breed. Ewes with exceptionally high prolificacy (hyper-prolific ewes) in current flocks on Irish farms were identified to simulate the screening of ewes from Irish flocks in the 1960s. DNA was obtained from: prolific ewes in extant flocks of Lleyn sheep (n = 44) on the Lleyn peninsula in Wales; hyper-prolific ewes (n = 41); prolific Galway (n = 41) ewes; Finnish Landrace (n = 124) and Texel (n = 19) ewes. The FecXG mutation was identified in Lleyn but not in Finnish Landrace, Galway or Texel sheep; FecXB was only found among the hyper-prolific ewes. The FecGH mutation was identified in the sample of Lleyn sheep. It was concluded from these findings that the Lleyn breed was the most likely source of the FecXG and FecGH mutations in Belclare and Cambridge sheep and that the FecXB mutation came from the High Fertility line that was developed using prolific ewes selected from commercial flocks in Ireland in the 1960′s and subsequently used in the genesis of the Belclare.Financial support through the Teagasc Walsh Fellowship Scheme, Genesis Faraday SPARK award (Lleyn survey) and Science Foundation Ireland (07/SRC/B1156) is gratefully acknowledged

An Assessment of the Economic, Environmental and Social Impacts of NSW Agriculture's Wheat Breeding Program

The Wagga wheat breeding program has been operating for over 100 years. In that time, it has released a flow of new wheat varieties for wheat growers in south-eastern Australia. Those varieties have led to increases in both yields and grain quality. The average annual rate of yield improvement in NSW has been 3.2% compared to the average for Australia of 2.4% with a significant proportion of these productivity gains arising from new varieties. In this analysis, the investment in that program from 1980 to 2003 has been evaluated. Given the lags inherent in wheat breeding investments, the benefits from those investments are being measured from 1993 to 2020. The broad structure of the program has remained relatively stable for most of the period since 1980. The program consists of 2-3 wheat breeders, one breeder-pathologist, and a cereal chemist, with appropriate technical and field support, totaling approximately 15 full-time equivalents per year. The costs of the program have averaged approximately $1.2 million per year over the period. In assessing the Wagga wheat breeding program it is important to consider how the industry would have developed without the program. The benefits of the program were measured as the difference in returns from improved wheat varieties in NSW over that period and the returns that would have been achieved in the absence of the Wagga breeding program. The assumption used to determine the impact without the Wagga program was that the rate of yield improvement in NSW would have been the same as for the rest of Australia. For quality, without the Wagga program the assumption was that in southern NSW the increase in quality would have been 20$321 million. The economic benefits of the breeding program are shared by producers, processors and consumers in the wheat industry, some of whom live overseas. Because Australia is largely a price taker on world wheat markets and because the wheat processing and distribution sector in Australia is generally considered to be competitive, most of the benefits of the wheat breeding program are likely to remain with producers. However these gains are offset by declines in the world price in response to advancing technology throughout the world. These economic benefits have positive social consequences, largely through their contribution to the incomes of farmers and those who handle and process wheat in regional NSW. Some of these gains are in the form of new marketing and processing industries around the increasingly specialised industry segments resulting directly from the changes that have occurred in wheat varieties. Perhaps these new skills add to the social capital of towns in the wheat belt of NSW. In environmental terms, the wheat breeding program itself is not likely to have major impacts, since the wheat industry would have been very similar whether or not there was a Wagga breeding program. However, to the extent that improved productivity from the Wagga program's varieties has allowed an expansion of the wheat industry, there could be some negative environmental consequences of the breeding program, such as those arising from the clearing of land, increased cultivation and increased use of herbicides. On the other hand, the high levels of disease resistance developed and maintained has meant that wheat production is not associated with large-scale fungicide use, and hence the danger of chemical contamination of the environment is less than it would have been without the resistance developed in this program. Some of these environmental impacts affect the costs and incomes of wheat farmers and hence are reflected in economic benefits and some spill over to the broader community and have not been valued here. It is not clear that these social and environmental impacts would be much different without the Wagga breeding program, except through the extent to which the Wagga program has allowed the wheat industry in NSW to develop more than it otherwise would have. Without the Wagga program the slower gains in yield and quality would also be associated with some social and environmental impacts, and it is the difference that is critical in evaluating the Wagga program. The costs of this program have been met partly by the NSW taxpayers through NSW Agriculture and partly by the grains industry through levies from the Grains Research and Development Corporation (GRDC). The recent introduction of variety royalty payments ("end-point royalties") has not yet led to significant funding, but may be expected to do so in the future. The nature of the outputs of plant breeding programs is that there are large economic benefits that flow directly to producers, processors and consumers in the industry. However the social and environmental impacts on the broader community, while not explicitly valued here, are considered to be small relative to economic benefits and relative to some other programs of NSW Agriculture that have been evaluated. Hence it is appropriate that the industry, though GRDC levies and royalties on production, has increasingly funded the operations of the wheat breeding program. Recent institutional changes for the wheat breeding program have made it even more commercially-based for the future and less reliant on government funding. The new institutional arrangements for wheat breeding programs and the strengthening role of the private sector in supplying varieties traditionally supplied by the public sector mean that the place of public wheat breeding programs is being re-assessed. A key question is whether publicly-operated programs, can offer some additional benefits either to the industry or to the community, which would not result from the complete privatisation of the wheat breeding sector. While those issues have not been addressed directly in this analysis, the results indicate that past investments in public wheat breeding program at Wagga have certainly been a productive use of public funds over the past 20 years or so.Research and Development/Tech Change/Emerging Technologies,